International Journal of Pediatrics

Induced Pluripotent Stem Cells: Challenges and Opportunities

Mina Shahriari; Zahra Hejazi; Moein Farshchian; Hojjat Naderi; Hamid Reza Bidkhori; Mahdi Mir-Ahmadi; Saeideh Nakhaei; Maliheh Hasan Zadeh; Mahmood Raees-Al-Mohaddesin; Asiyeh Heirani; Maryam M. Matin; Ahmad Reza Bahrami

Volume 2, Issue 2.3 , May 2014, , Pages 13-13

https://doi.org/10.22038/ijp.2014.2662

Abstract

Regenerative capacity of mammals is limited and can rarely regenerate a specific organ or tissue fully. Due to these limitations, regenerative medicine seeks efficient and safe cell ... Read More

Comparative analysis of the Gene expression profile of Chemokine Receptors between Adipose-derived and Bone marrow-derived Mesenchymal Stem Cells

Hamid Reza Bidkhori; Moien Farshchian; Asieh Heirani-Tabasi; Hojjat Naderi-Meshkin1; Mahtab Dastpak; Naghmeh Ahmadian Kia; Ahmad Reza Bahrami; Maryam M. Matin

Volume 2, Issue 2.3 , May 2014, , Pages 17-17

https://doi.org/10.22038/ijp.2014.2666

Abstract

Introduction: Mesenchymal stem cells (MSCs) hold great promise in the field of regenerative medicine.Although originally isolated from bone marrow, MSCs have since been obtained from ... Read More

Strategies to Improve Homing of Stem Cells to achieve better Efficacy in Stem Cell Therapy

Hojjat Naderi-Meshkin; Ahmad Reza Bahrami; Hamid Reza Bidkhori; Mahdi Mirahmadi; Naghmeh Ahmadiankia

Volume 2, Issue 2.3 , May 2014, , Pages 18-18

https://doi.org/10.22038/ijp.2014.2667

Abstract

Stem/progenitor cell based therapeutic approach in our daily routine clinical practice, has been elusive dream in medical sciences and improvement of stem cell homing as one of major ... Read More Stem/progenitor cell based therapeutic approach in our daily routine clinical practice, has been elusive dream in medical sciences and improvement of stem cell homing as one of major challenges in cell therapy programs, has been considered a promising milestone. It has been proved that stem/progenitor cells exhibit a homing response to injured tissues/organs, mediated by interactions of chemokine receptors expressed on the cells and chemokines secreted by the injured tissue. For improvement of directed homing of the cells, many techniques have been developed either to engineer stem/progenitor cells with higher amount of chemokine receptors (stem cell-based strategies) or to modulate the target tissues to release higher level of the corresponding chemokines (target tissue-based strategies). Treatment with chemical compounds, preconditioning of the cells with hypoxia, cytokine and growth factor priming of the cells, genetic modifications, coating of cell surface with antibodies, glycoengineering, coating of stem cells with homing ligands by streptavidin linkers are some of strategies to increase the ability of stem cells to respond to the migratory stimuli. On the other side to modulate target sites to be more attractive for stem cells, some strategies like direct injection of chemokines, direct transfection of the target tissue with chemokine encoding genes, injection of ectopic chemokine expressing cells, application of scaffolds, electrical fields and low level laser have been introduced. These extensive investigations have provided significant potentials to enhance targeted stem/progenitor cells homing. Meanwhile there are still some limitations to apply these findings in clinics. To overcome these limitations, further studies should be aimed, unveiling the molecular and cellular mechanisms underlying endogenous cell trafficking during physiological and pathological events like embryogenesis, inflammation, wound healing, or cancer metastasis. Keywords: Cell therapy, Homing, Stem cells.

Pretreatment of Mesenchymal Stem Cells and Stromal-derived Factor-1α Delivery from Chitosan-based Injectable Hydrogels for Better Cell Guidance and Retention

Hojjat Naderi-Meshkin; Maryam M. Matin; Asieh Heirani-Tabasi; Maliheh Hasanzadeh; Mina Shahryari; Naghmeh Ahmadiankia; Mahmood Raisolmohaddesin; Nasser Sanjar Moussavi; Hamid Reza Bidkhori; Mahmoud Hosseini; Ahmad Reza Bahrami

Volume 2, Issue 2.3 , May 2014, , Pages 19-19

https://doi.org/10.22038/ijp.2014.2668

Abstract

Clinical applications of mesenchymal stem cells (MSCs) rely on their capacity to home and engraft in the appropriate target tissues for a long time. Homing and engraftment capacity ... Read More Clinical applications of mesenchymal stem cells (MSCs) rely on their capacity to home and engraft in the appropriate target tissues for a long time. Homing and engraftment capacity of these stem cells depend on the expression of Chemokines and their receptors. Ex vivo expanded MSCs exhibit homing potential when grafted to injury tissue but their homing efficiency has been observed very poor because of modifications in homing receptor expression and/or functions during culture and/or preparation steps. Hence, this study was designed to investigate the expression of surface CXCR4 by flow cytometric analysis (FACS) and in vitro modified Boyden chamber assay in adipose-derive MSCs (ASCs) stimulated with a hypoxia mimicking agents such as desferrioxamine mesilate (DFX), cobalt chloride (CoCl2), lithium chloride (LiCl), valproic acid (VPA) and hypoxia. Intracellular CXCR4 were also evaluated by conventional and real-time PCR. Then we evaluated the homing ability of DFX-pretreated human DiI-labeled ASCs in vivo, 2 weeks after intravenous (IV), local infusion towards subcutaneously implanted chitosan-glycerophophate-hydroxyethyl cellulose (CH-GP-HEC) injectable hydrogels releasing SDF1 in dorsum of Wistar Rats. Presence of human ASCs in the CH-GP-HEC injectable, spleen, and lung were analyzed histologically by fluorescent microscope, and also quantified by PCR for human specific CXCR4 gene, 2 weeks after transplantation in recipients' Rats. Results showed that short-term (24 hours) pretreatment to ASCs with the hypoxia mimicking agents up-regulate the CXCR4, increase in vitro migration capacity toward 100ng/ml SDF-1 (P<0.001) and in vivo homing capacity to the implanted CH-GP-HEC injectable hydrogel releasing SDF1. Fluorescence microscopic examination disclosed enhanced local accumulation of fluorescence-labeled ASCs in CH-GP-HEC in the DFX-pretreated group at 16th post-transplantation day. These results suggest that the SDF-1/CXCR4 axis plays an important role in the regulation of motility of ASCs, and increased expression of CXCR4 might be a potential strategy to improve homing and engraftment of ASCs towards SDF1 released by injectable hydrogels in different lesions. Keywords: Chemotactic recruitment, Guided homing, Stem cells therapy.

Stem Cell-Based Therapy (Cell Therapy) in Iran and other Countries

Mohammad Mousaei Ghasroldasht; Maryam M.Matin; Hamid Reza Bidkhori; Ahmad Reza Bahrami

Volume 2, Issue 2.3 , May 2014, , Pages 63-63

https://doi.org/10.22038/ijp.2014.2726

Abstract

Cell therapy is a modern approach in the treatment of most of the diseases which uses stem cells and it has been recently developed rapidly. Cell therapy has shown satisfactory results ... Read More

International Journal of Pediatrics

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Author = Hamid Reza Bidkhori

Induced Pluripotent Stem Cells: Challenges and Opportunities

Abstract

Comparative analysis of the Gene expression profile of Chemokine Receptors between Adipose-derived and Bone marrow-derived Mesenchymal Stem Cells

Abstract

Strategies to Improve Homing of Stem Cells to achieve better Efficacy in Stem Cell Therapy

Abstract

Pretreatment of Mesenchymal Stem Cells and Stromal-derived Factor-1α Delivery from Chitosan-based Injectable Hydrogels for Better Cell Guidance and Retention

Abstract

Stem Cell-Based Therapy (Cell Therapy) in Iran and other Countries

Abstract