International Journal of Pediatrics

Induced Pluripotent Stem Cells: Challenges and Opportunities

Mina Shahriari; Zahra Hejazi; Moein Farshchian; Hojjat Naderi; Hamid Reza Bidkhori; Mahdi Mir-Ahmadi; Saeideh Nakhaei; Maliheh Hasan Zadeh; Mahmood Raees-Al-Mohaddesin; Asiyeh Heirani; Maryam M. Matin; Ahmad Reza Bahrami

Volume 2, Issue 2.3 , May 2014, , Pages 13-13

https://doi.org/10.22038/ijp.2014.2662

Abstract

Regenerative capacity of mammals is limited and can rarely regenerate a specific organ or tissue fully. Due to these limitations, regenerative medicine seeks efficient and safe cell ... Read More

Genetic and Epigenetic landscape of Germline Stem Cells

Moein Farshchian; Mahtab Dastpak; Maryam M. Matin; Dirk Geerts; Ahmad Reza Bahrami

Volume 2, Issue 2.3 , May 2014, , Pages 14-14

https://doi.org/10.22038/ijp.2014.2663

Abstract

Elucidating the critical epigenetics events involved in differentiation and reprogramming of cells to primordial germ cells (PGCs) is among the interesting issues in stem cell research. Here, ... Read More

Comparative analysis of the Gene expression profile of Chemokine Receptors between Adipose-derived and Bone marrow-derived Mesenchymal Stem Cells

Hamid Reza Bidkhori; Moien Farshchian; Asieh Heirani-Tabasi; Hojjat Naderi-Meshkin1; Mahtab Dastpak; Naghmeh Ahmadian Kia; Ahmad Reza Bahrami; Maryam M. Matin

Volume 2, Issue 2.3 , May 2014, , Pages 17-17

https://doi.org/10.22038/ijp.2014.2666

Abstract

Introduction: Mesenchymal stem cells (MSCs) hold great promise in the field of regenerative medicine.Although originally isolated from bone marrow, MSCs have since been obtained from ... Read More

Pretreatment of Mesenchymal Stem Cells and Stromal-derived Factor-1α Delivery from Chitosan-based Injectable Hydrogels for Better Cell Guidance and Retention

Hojjat Naderi-Meshkin; Maryam M. Matin; Asieh Heirani-Tabasi; Maliheh Hasanzadeh; Mina Shahryari; Naghmeh Ahmadiankia; Mahmood Raisolmohaddesin; Nasser Sanjar Moussavi; Hamid Reza Bidkhori; Mahmoud Hosseini; Ahmad Reza Bahrami

Volume 2, Issue 2.3 , May 2014, , Pages 19-19

https://doi.org/10.22038/ijp.2014.2668

Abstract

Clinical applications of mesenchymal stem cells (MSCs) rely on their capacity to home and engraft in the appropriate target tissues for a long time. Homing and engraftment capacity ... Read More Clinical applications of mesenchymal stem cells (MSCs) rely on their capacity to home and engraft in the appropriate target tissues for a long time. Homing and engraftment capacity of these stem cells depend on the expression of Chemokines and their receptors. Ex vivo expanded MSCs exhibit homing potential when grafted to injury tissue but their homing efficiency has been observed very poor because of modifications in homing receptor expression and/or functions during culture and/or preparation steps. Hence, this study was designed to investigate the expression of surface CXCR4 by flow cytometric analysis (FACS) and in vitro modified Boyden chamber assay in adipose-derive MSCs (ASCs) stimulated with a hypoxia mimicking agents such as desferrioxamine mesilate (DFX), cobalt chloride (CoCl2), lithium chloride (LiCl), valproic acid (VPA) and hypoxia. Intracellular CXCR4 were also evaluated by conventional and real-time PCR. Then we evaluated the homing ability of DFX-pretreated human DiI-labeled ASCs in vivo, 2 weeks after intravenous (IV), local infusion towards subcutaneously implanted chitosan-glycerophophate-hydroxyethyl cellulose (CH-GP-HEC) injectable hydrogels releasing SDF1 in dorsum of Wistar Rats. Presence of human ASCs in the CH-GP-HEC injectable, spleen, and lung were analyzed histologically by fluorescent microscope, and also quantified by PCR for human specific CXCR4 gene, 2 weeks after transplantation in recipients' Rats. Results showed that short-term (24 hours) pretreatment to ASCs with the hypoxia mimicking agents up-regulate the CXCR4, increase in vitro migration capacity toward 100ng/ml SDF-1 (P<0.001) and in vivo homing capacity to the implanted CH-GP-HEC injectable hydrogel releasing SDF1. Fluorescence microscopic examination disclosed enhanced local accumulation of fluorescence-labeled ASCs in CH-GP-HEC in the DFX-pretreated group at 16th post-transplantation day. These results suggest that the SDF-1/CXCR4 axis plays an important role in the regulation of motility of ASCs, and increased expression of CXCR4 might be a potential strategy to improve homing and engraftment of ASCs towards SDF1 released by injectable hydrogels in different lesions. Keywords: Chemotactic recruitment, Guided homing, Stem cells therapy.

International Journal of Pediatrics

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Author = Maryam M. Matin

Induced Pluripotent Stem Cells: Challenges and Opportunities

Abstract

Genetic and Epigenetic landscape of Germline Stem Cells

Abstract

Comparative analysis of the Gene expression profile of Chemokine Receptors between Adipose-derived and Bone marrow-derived Mesenchymal Stem Cells

Abstract

Pretreatment of Mesenchymal Stem Cells and Stromal-derived Factor-1α Delivery from Chitosan-based Injectable Hydrogels for Better Cell Guidance and Retention

Abstract