International Journal of Pediatrics

In Cadaver kidney Recipients, Autologous Bone Marrow Stem Cell Transplantation Significantly Improve Graft Function, Short-term Outcome

Daryoush Hamidi Alamdari; Farzaneh Sharifipour; Hassan Ravari; Fereshteh Mamdouhi; Massih Naghibi; Maryam Hami; Abbasali Zeraati; Fatemeh Nazemian; Reza Mahdavi; Mahmood Tavakoli; Ebrahim Khaleghi

Volume 2, Issue 2.3 , May 2014, , Pages 28-28

https://doi.org/10.22038/ijp.2014.2677

Abstract

Background: Renal transplantation is the best choose in the end stage renal disease (ESRD), and acute rejection and graft dysfunction remain major challenges in the worldwide, ... Read More Background: Renal transplantation is the best choose in the end stage renal disease (ESRD), and acute rejection and graft dysfunction remain major challenges in the worldwide, even with the advent of new immunosuppressive drugs. The novel cell-based anti-rejection treatments have been studied by using different stem cell sources. In this study, transplantation of autologous-bone-marrow-derived-total-nucleated-cells is used to improve the cadaver kidney graft function. Methods: 18 ESRD patients, which candidate for cadaver kidney transplantation, were divided in two groups (group A and B) and there was no significant difference (male, female, age, weight, type of dialysis) between two groups. The two kidney of one cadaver were transplanted to two recipients. Before transplantation, randomly, autologous-bone-marrow aspiration was done for one recipient, and then the transplantation was done for both of recipients. The total-nucleated-cells were separated and infused intravenously during and after the transplantation, respectively. In post transplantation, 6 hours diuresis, delay graft function (DGF), creatinine (1 week, 2 weeks and 3 months), cyclosporine (2 weeks) and cold ischemia were measured in bone-marrow-treated group (A: 9 patients) and non-bone-marrow-treated group (B: 9 patients). Results: A significant increase in diuresis, a significant decrease in DGF, a significant decrease (1 week and 2 weeks) and marginally significant decrease (3 month) in creatinine, no significant difference in cyclosporine blood level (2 weeks), operation time and cold ischemia were seen in group A in comparison to group B. 2 patients had CMV infection in group B, but none in group A. Conclusion: The transplantation of autologous-bone-marrow-cells in cadaver kidney recipient is safe and significantly decreases early graft dysfunction. Further clinical trial is needed to confirm these promising results. Keywords: Bone marrow, Delayed graft function, Kidney transplantation, Stem cell.

A Novel Method for Iatrogenic Vesicovaginal Fistula Treatment: Autologous Platelet Rich Plasma Injection and Platelet Rich Fibrin Glue Interposition

Maliheh Keshvari Shirvan; Daryoush Hamidi Alamdari; Alireza Ghoreifi

Volume 2, Issue 2.3 , May 2014, , Pages 29-29

https://doi.org/10.22038/ijp.2014.2678

Abstract

Introduction: Vesicovaginal fistula remains a challenge in surgical therapy. In this study autologous platelet rich plasma and platelet rich fibrin glue were used as a minimally invasive ... Read More

Human Mesenchymal Stem Cells Derived from Adiopose Tissue and Placenta and the Adipocytic and Osteocytic Differentiation

Zahra Mohammadi; Jalil Tavakol Afshari; Mohammad Reza Keramati; Daryoush Hamidi Alamdari; Meysam Ganjibakhsh; Azam Moradi Zarmehri; Ali Jangjoo; Mohammad Hadi Sadeghian

Volume 2, Issue 2.3 , May 2014, , Pages 39-39

https://doi.org/10.22038/ijp.2014.2692

Abstract

Introduction: Mesenchymal stem cells can be isolated from adult tissues, such as the adipose tissue, or other sources. Among all these sources, adipose tissue because of easy access, ... Read More

Histological Evidences after Platelet-Rich-Plasma and Adipose Drived Stem Cells Injection on Critical Size Cleft Palate

Sima Tavakolinejad; Daryoush Hamidi Alamdari; Saeedeh Khajehahmadi; Alireza Ebrahimzadeh Bidskan

Volume 2, Issue 2.3 , May 2014, , Pages 88-88

https://doi.org/10.22038/ijp.2014.2762

Abstract

Background Cleft palate (CP) is a common congenital defect. It makes serious difficulties for cleft-affected children. The gold standard of care is autogenous bone grafting which may ... Read More Background Cleft palate (CP) is a common congenital defect. It makes serious difficulties for cleft-affected children. The gold standard of care is autogenous bone grafting which may cause additional problems in donor site along with disappointing results. Tissue engineering is a promising solution for a widespread range of defects and disorders. It is reasonable to utilize this novel technology for CP management. Stem cells and growth factors play essential role in tissue engineering. Adipose tissue contains a population of stem cells that can be isolated and differentiated into various cell lines, including osteoblasts. In this study, the authors used human Adipose-derived stem cells(hADSCs) and osteogenically differentiated hADSCs along with platelet rich plasma(PRP), a source of growth factors, to repair rat palatal bone defects. Materials and Method: Palatal bone defects were surgically made in 56 female rats. Animals divided into 7 groups (n=8). Human adipose-derived stem cells were collected and incubated with Bromodeoxyuridine (BrdU) in order to labeling. The same was done to osteogenically differentiated hADSCs. Afterwards, the labeled cells were mixed either with PRP or Aminoplasmal and injected to the defect borders. Immunohistochemistry and morphometry analysis were performed 4weeks later. Results: Data showed a significant difference in cleft size between cell-injected and control groups while the cleft site fills with connective tissue rather than osseous tissue. Moreover, immunohistochemistry findings proved the presences of labeled cells in surrounding tissue. There is no significant difference between undifferentiated and osteogenically differentiated cells both in numerical area density of cells and defect size. Conclusion: This study revealed the feasibility of stem cell and PRP application according to CP reconstruction. Further investigation toward clinical application of tissue engineering in CP may eliminate bone harvesting and its negative consequences. Keywords: Adipose Derived Stem Cells, Cell Therapy, Cleft Palate, Platelet-Rich-Plasma.

International Journal of Pediatrics

Articles in Press

Current Issue

Volume 12 (2024)

Volume 11 (2023)

Volume 10 (2022)

Volume 9 (2021)

Volume 8 (2020)

Volume 7 (2019)

Volume 6 (2018)

Volume 5 (2017)

Volume 4 (2016)

Volume 3 (2015)

Volume 2 (2014)

Volume 1 (2013)

Author = Daryoush Hamidi Alamdari

In Cadaver kidney Recipients, Autologous Bone Marrow Stem Cell Transplantation Significantly Improve Graft Function, Short-term Outcome

Abstract

A Novel Method for Iatrogenic Vesicovaginal Fistula Treatment: Autologous Platelet Rich Plasma Injection and Platelet Rich Fibrin Glue Interposition

Abstract

Human Mesenchymal Stem Cells Derived from Adiopose Tissue and Placenta and the Adipocytic and Osteocytic Differentiation

Abstract

Histological Evidences after Platelet-Rich-Plasma and Adipose Drived Stem Cells Injection on Critical Size Cleft Palate

Abstract