International Journal of Pediatrics

Leukemia- Derived Exosomes Activate Migration and Tumor-Associated Genes in Astrocytes Isolated from Human Brain Tissue

Parisa Zamani Esmati; Javad Baharara; Sajad Sahab Negah; Khadijeh Nejad Shahrokhabadi

Volume 9, Issue 10 , October 2021, , Pages 14540-14548

https://doi.org/10.22038/ijp.2021.52934.4192

Abstract

Background: In children with lymphoblastic leukemia (ALL), cancerous metastasis to the central nervous system (CNS) is common, but there is insufficient information on this metastasis ... Read More Background: In children with lymphoblastic leukemia (ALL), cancerous metastasis to the central nervous system (CNS) is common, but there is insufficient information on this metastasis and cancer progression process. Further, the role of exosomes on cancer growth and metastasis has been the attention of many studies. Because the astrocytes involve in the blood-brain barrier and stay in contact with peripheral blood , we investigated the effect of exosomes derived Nalm6 cell line on astrocytes from human brain fetal tissue. Methods: In this in vitro study, exosomes were isolated from the supernatant of Nalm6 cell line by centrifugation and identified by DLS, AFM and FE-SEM methods. Astrocytes were isolated from human fetal brain tissue cells and expanded and identified by GFAP antibody with immunocytochemistry. Astrocytes were treated with different concentrations of exosomes (i.e., 5, 10, 30, and 50 µg/ml),. Proliferation and migration were assessed with Trypan blue staining and scratch methods, respectively. To define tumorogensis changes in astrocytes treated by exosomes, the expression of MMP9, P53, and Cox2 genes were investigated by Real-Time PCR.Findings: According to the DLS results, the size of exosomes was about 43 nm. AFM and FE-SEM imaging indicated these exosomes have a spherical shape. Our results showed that the proliferation of astrocytes significantly increased when treated with 50 µg/ml of exosomes.Therefore, the concentration of 50 µg/ml was considered for further experiments. Astrocyte migration was significantly increased compared to the control group. Finally, our results showed tumorogenesis changes in astrocytes by increasing the expression of MMP9 and Cox2 and decreasing the expression of p53 at mRNA level.Conclusion: Based on our in vitro study, exosomes derived from ALL as a peripheral tumor can change the behavior of astrocytes as a target for metastasis. Further investigations on this topic are warranted to shed light on metastatic mechanisms.

Modifiable and Non-Modifiable Factors Affecting the Risk of Childhood Leukemia: An Overview of Meta-Analysis

Rana Tafrishi; Benyamin Seyfari; Rahele Rahimi; Zahra Chaichi; Amirreza Dehghan Tarazjani; Nahid Marvi; Mahbubeh Maazallahi; Zari Dolatian; Farzane Ashrafinia

Volume 9, Issue 3 , March 2021, , Pages 13243-13248

https://doi.org/10.22038/ijp.2020.48245.3888

Abstract

Background: Numerous meta-analyses of childhood leukemia have been published, but no comprehensive study has examined the factors influencing childhood leukemia. We aimed to scrutinize ... Read More

Exposure to Magnetic Fields and Childhood Leukemia: An Overview of Meta-Analysis

Sara Ghahremani; Khatereh Shiroudbakhshi; Amir Hossein Salimi Kordasiabi; Mohammad FiroozBakht; Mohsen Hosseinzadegan; Farzane Ashrafinia; Sara Rahafard

Volume 8, Issue 5 , May 2020, , Pages 11361-11365

https://doi.org/10.22038/ijp.2020.47472.3851

Abstract

Background: Leukemia is the most common cancer in children. We aimed to evaluate the association between exposure to magnetic fields and childhood leukemia. Materials and Methods: In ... Read More

Comparison of CNS Relapse, Survival and Intelligent Quotient in Non-High Risk ALL Children Treated with Intrathecal Methotrexate or Triple Intrathecal Therapy

Nahid Raeissi; Alireza Moafi; Narges Alikhasi

Volume 7, Issue 8 , August 2019, , Pages 9955-9965

https://doi.org/10.22038/ijp.2019.40438.3419

Abstract

Background Compared to intrathecal methotrexate (IT MTX), triple intrathecal therapy (TIT) has shown promising results in decreasing central nervous system (CNS) relapse in children ... Read More

Care Burden in Parents of Children with Leukemia: A Cross-Sectional Study in the West of Iran

Mohammad Esmail Motlagh; Mehdi Mirzaei-Alavijeh; Seyyed Nasrollah Hosseini

Volume 7, Issue 6 , June 2019, , Pages 9541-9548

https://doi.org/10.22038/ijp.2019.38584.3305

Abstract

Background Parental caregiver burden is an important priority in children's oncology researches. The present study aimed to determine care burden among parents of children with leukemia. ... Read More

Efficacy of Acupressure on Nausea and Vomiting in Children Undergoing Chemotherapy: A Systematic Review

Mohadeseh Khakpour; Babak Dabbaghi; Sepideh Noorkhomami; Asma Javid; Majid Sezavar; Masumeh Ghazanfarpour

Volume 7, Issue 6 , June 2019, , Pages 9601-9610

https://doi.org/10.22038/ijp.2019.39206.3338

Abstract

Background: Chemotherapy is the main component of systemic cancer treatment; however, the effective application is restricted due to nausea and vomiting. We aimed to assess the effect ... Read More

The Effect of Resilience Training on the Condition of Style of Coping and Parental Stress in Mothers of Children with Leukemia

Marziyeh Kaboudi; Parvin Abbasi; Parisa Heidarisharaf; Fateme Dehghan; Arash Ziapour

Volume 6, Issue 3 , March 2018, , Pages 7299-7310

https://doi.org/10.22038/ijp.2018.29245.2559

Abstract

Background Diagnosis of cancer in children has an adverse effect on the family. Resilience refers to the ability of humans to adapt to diseases, the pain and suffering caused by parental ... Read More

Complementary and Alternative Medicine in the Pediatrics with Leukemia: A Narrative Review Study

Mojtaba Miladinia; Zohore Lamoochi; Parisa Lamoochi; Kourosh Zarea; Marzieh Shayesteh Fard; Hojjat zareh Houshyari Khah

Volume 5, Issue 1 , January 2017, , Pages 4175-4183

https://doi.org/10.22038/ijp.2016.7324

Abstract

BackgroundFor using of complementary and alternative medicine methods (CAM) in the leukemia pediatrics than other types of cancer, we have two great challenges; first challenge is their ... Read More

Anxiety Control in the Iranian Children with Chronic Leukemia: Use of a Non-drug Method

Mojtaba Miladinia; Leila Fakharzadeh; Kourosh Zarea; Elham Mousavi Nouri

Volume 4, Issue 1 , January 2016, , Pages 1225-1231

https://doi.org/10.22038/ijp.2016.6307

Abstract

Background Among all malignances, leukemia has the greatest effects on patients 'psychological aspects. Anxiety is common problem in leukemia patients (especially in children). Since ... Read More

Effects of Slow-stroke Back Massage on Chemotherapy-induced Nausea and Vomiting in the Pediatrics with Acute Leukemia: a Challenge of Controlling Symptoms

Mojtaba Miladinia; Shahram Baraz; Elham Mousavi Nouri; Mehdi Gholamzadeh Baeis

Volume 3, Issue 6.2 , December 2015, , Pages 1145-1152

https://doi.org/10.22038/ijp.2015.6134

Abstract

Introduction Nausea and vomiting are the most common side effects of chemotherapy in the pediatrics with cancer which affect their quality of life. Use of some methods of complementary ... Read More Introduction Nausea and vomiting are the most common side effects of chemotherapy in the pediatrics with cancer which affect their quality of life. Use of some methods of complementary medicine in leukemia patients is problematic. Because, leukemia patients are at risk of infection and bleeding, therefore the use of acupressure, acupuncture, and deep massage can be risky in these patients. Slow- stroke back massage is applied on the surface of body, so does not have complications. No study has addressed the effect of massage therapy on chemotherapy-induced nausea and vomiting in pediatrics with acute leukemia in the world. Material and methods This study was a two-group randomized controlled trial (RCT), double blind and repeated measures design. In this RCT, 45 school age children with acute leukemia were placed in the massage and control groups. Before start of the study, at the day of chemotherapy administration (day 1th), only nausea and vomiting were measured. Then during 6 days next (day 2 through 7), the intervention group received 5-minutes Super Smash Bros. Melee (SSBM), immediately before start of each session of chemotherapy. Nausea was measured during chemotherapy, 0.5 h and 3 h after each session of chemotherapy in the two groups. Also vomiting was recorded during 24 h after each session of chemotherapy. Repeated measures ANOVA, Chi-square, and t-test were used for analysis. Results Most of pediatrics were male (58.13%), and suffered from Acute myeloid leukemia (AML) (81.7%). The repeated measure analysis showed that in the intervention group, the SSBM reduced progressive mean of nausea severity and frequency of vomit over time. While, this side effects have slightly increased over time in the control group. Conclusion The results of this study are suggesting that SSBM, as a non-pharmacologic, easy and safe method, is effective in controlling Chemotherapy-induced nausea and vomiting (CINV) in the pediatrics with acute leukemia.

International Journal of Pediatrics

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Keywords = Leukemia

Leukemia- Derived Exosomes Activate Migration and Tumor-Associated Genes in Astrocytes Isolated from Human Brain Tissue

Abstract

Modifiable and Non-Modifiable Factors Affecting the Risk of Childhood Leukemia: An Overview of Meta-Analysis

Abstract

Exposure to Magnetic Fields and Childhood Leukemia: An Overview of Meta-Analysis

Abstract

Comparison of CNS Relapse, Survival and Intelligent Quotient in Non-High Risk ALL Children Treated with Intrathecal Methotrexate or Triple Intrathecal Therapy

Abstract

Care Burden in Parents of Children with Leukemia: A Cross-Sectional Study in the West of Iran

Abstract

Efficacy of Acupressure on Nausea and Vomiting in Children Undergoing Chemotherapy: A Systematic Review

Abstract

The Effect of Resilience Training on the Condition of Style of Coping and Parental Stress in Mothers of Children with Leukemia

Abstract

Complementary and Alternative Medicine in the Pediatrics with Leukemia: A Narrative Review Study

Abstract

Anxiety Control in the Iranian Children with Chronic Leukemia: Use of a Non-drug Method

Abstract

Effects of Slow-stroke Back Massage on Chemotherapy-induced Nausea and Vomiting in the Pediatrics with Acute Leukemia: a Challenge of Controlling Symptoms

Abstract