@article { author = {Farshchian, Moein and Dastpak, Mahtab and M. Matin, Maryam and Geerts, Dirk and Bahrami, Ahmad Reza}, title = {Genetic and Epigenetic landscape of Germline Stem Cells}, journal = {International Journal of Pediatrics}, volume = {2}, number = {2.3}, pages = {14-14}, year = {2014}, publisher = {Mashhad University of Medical Sciences}, issn = {2345-5047}, eissn = {2345-5055}, doi = {10.22038/ijp.2014.2663}, abstract = {Elucidating the critical epigenetics events involved in differentiation and reprogramming of cells to primordial germ cells (PGCs) is among the interesting issues in stem cell research. Here, I will talk about critical transcription factors and global hypomethylation in development of germ cells. Evidence strongly suggests that the earliest PGCs emerging in the E7.25 mouse embryo epiblast have a highly methylated genome, and high level of H3K9me2 in chromatin but during development, genome demethylated and patterns of histone codes changes dramatically. We designed a polycistroniclentiviral vector and overexpressed Stella, Oct4 and Nanos2 simultaneously in transduced cells; Increasing level of Prdm14, Nanog and decreasing of G9a expression is an interesting finding which might be considered as a primary step of reprogramming toward germline progenitor cells, here we propose decreasing H3K9me2 level as a consequence of G9a down regulation is a critical step which facilitated transition to different stemness state through creating a new epigenetic memory for the early germ cells.   Keywords: Epigenetic, hypomethylation, Germ line Stem Cells, polycistroniclentiviral vector.  }, keywords = {Oral Presentation}, url = {https://ijp.mums.ac.ir/article_2663.html}, eprint = {https://ijp.mums.ac.ir/article_2663_1d4f86e61f0f63dd9293424cae79d78d.pdf} }