The Survey of DBH Gene Polymorphism Rs5320 in Children with Attention Deficit Hyperactivity Disorder (ADHD)

Authors

1 Professor of Child and Adolescent Psychiatry, Research Center of Psychiatry and Behavioral Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.

2 Department of Physiology, Tabriz Branch, Islamic Azad University, Tabriz, Iran.

3 Associate Professor of Psychiatry, Research Center of Psychiatry and Behavioral Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.

4 General Practitioner, Research Center of Psychiatry and Behavioral Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.

5 Psychologist, Research Center of Psychiatry and Behavioral Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.

6 Cognitive Neuroscience, Department of Biology, University of Tabriz, 29 Bahman Bolvard, Tabriz, Iran.

Abstract

Background
Attention deficit hyperactivity disorder (ADHD) is a common behavioral disorder that affects 8-12% of school-age children. Several environmental and genetic factors play a role in the etiology of this disease. One of the genetic factors involved is dopamine beta-hydroxylase (DBH) gene, which plays an essential role in catecholamine synthesis by converting dopamine into norepinephrine. Here we investigated DBH polymorphisms associated with ADHD in North West of Iran.
Materials and Methods: This descriptive comparative study was performed on 130 children aged 5-14 years who were diagnosed with ADHD by child and Adolescent psychiatrist following a detailed psychiatric assessment and 130 matching healthy children were also selected from local children’s Hospital in Tabriz city, Iran. Also, 2ml Peripheral blood sample was obtained from all the participants and RFLP-PCR technique was then used to study the polymorphism position rs5320 and allele and genotype frequency of DBH gene.
Results: The results showed that the frequency of allele A (as the allele causing the disorder) was 15% in ADHD subjects and 6% in healthy subjects (p <0.05). The genotype frequency in ADHD subjects was 4%AA, 26%AG, and 70%GG, and 0%, 12% and 88% for healthy children, respectively (p=0.017, do=2, χ2=3.14).
Conclusion: The results suggest that DBH polymorphism, position rs5320, plays a role in the pathogenicity of ADHD in the studied population and therefore can be considered as a candidate gene for future diagnosis.

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