Evaluation of some caries-related factors in the saliva of 3 to 5 years old children in Sari (Northern Iran)

Authors

1 Pharmaceutical Science Research Center, Hemoglobinopathy Institute, Mazandaran University of Medical Sciences, Sari, Iran AND Assistant Professor, Department of Toxicology and Pharmacology, Faculty of Pharmacy, Mazandaran University of Medical Sciences, Sari, Iran.

2 PhD Candidate in Toxicology, Student Research Committee, Mazandaran University of Medical Sciences, Sari, Iran.

3 Student of Dentistry, Department of Pediatric Dentistry, Faculty of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran.

4 Professor, Department of Biostatistics, School of Health Sciences, Mazandaran University of Medical Sciences, Sari, Iran.

5 Assistant Professor, Department of Pediatric Dentistry, Faculty of Dentistry, Mazandaran University of Medical Sciences, Sari, Iran.

Abstract

Abstract
Background: Dental caries is one of the most incidence oral disease in pre-school children. Several factors can affect caries process. Aim of this study was comparison of the some chemical properties of saliva such as total antioxidant capacity, total protein, pH, nitric oxide level in caries free (CF), and caries active (CA) children.
Methods: This cross-sectional based study was designed with random selection of 80 healthy population including 40 CF and 40 CA children (3 to 5 years old) from several public kindergartens in Sari, Iran in year 2019. Caries status was assessed using DMFT (Decayed/Missing/Filled Teeth) index according to WHO criteria. Un-stimulated saliva samples were collected from children at the morning. Then, several caries-related factors including total antioxidant capacity, nitric oxide, total protein concentration and pH were assessed in saliva samples. Data were analyzed by SPSS14 software using student’s t-test.
Results: Significant higher total antioxidant capacity and total protein concentration were observed in the saliva of C.A than in the C.F children. On the other hand, nitric oxide level was markedly lower in C.A samples. Also, a decrease in pH of saliva was observed in C.A children.
Conclusion: According to these results, increase in the total antioxidant capacity and total protein as well as decrease in nitric oxide levels in the saliva of C.A children can be considered as valuable evidences of dental caries occurrence among children.

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