The relationship of serum levels of gamma interferon and its receptor (CD119) to development of BCG axillary lymphadenopathy

Authors

1 Assistant Professor, Department of Pediatrics, Shahrekord University of Medical Sciences, Shahrekord, Iran.

2 Department of Pediatrics, Shahrekord University of Medical Sciences, Shahrekord, Iran.

3 Department of Microbiology and Immunology, Shahrekord University of Medical Sciences, Shahrekord, Iran.

4 Professor, Department of Biostatistics and Epidemiology, Faculty of Health, Shahrekord University of Medical Sciences, Shahrekord, Iran.

5 Medical Student, Shiraz University of Medical Sciences, Shiraz, Iran, Address: Shiraz University of Medical Sciences, Medical school, Shiraz, Iran.

6 Professor, Department of Pediatrics, Clinical Search Department Unit, Hajar Hospital, Shahrekord University of Medical Sciences, Shahrekord, Iran.

Abstract

Background and Aim: Lymphadenitis is the most common complication of BCG vaccination in children. Interferon-gamma (IFN-γ) plays a key role in immune response to Mycobacterial infections. In this study, the relationship of serum levels of IFN-γ and its receptor (CD119) to development of BCG axillary lymphadenopathy was investigated.
Materials and methods: In this case-control study, 45 children with axillary lymphadenopathy and 45 healthy children matched by age and sex were included. 2 ml peripheral blood was collected in tubes containing anticoagulants. Then, level of IFN-γ was measured by ELISA and the level of CD119 expression in the peripheral blood mononuclear cell (PBMC) by flow cytometry. Data was analyzed by SPSSv22.
Results: Totally, 90 children were enrolled in this study which consisted of 30 girls and 60 boys. The mean age of participants was 14.5±6.5 months in case group and 15.2±7.1 months in control group (P=0.61). The level of IFN-γ was significantly lower in case group than in control group (p<0.001), but no significant difference was observed in PBMC percentage between the two groups (P>0.05). IFN-γ and PBMC were not significantly associated with age and sex (P>0.05).
Conclusion: IFN-γ level was significantly lower in the BCG lymphadenopathy group than in the control group. Levels of IFN-γR (CD119 cellular level in PBMC) in two groups did not show a significant relationship.

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