An Association Analysis of Reelin Gene (RELN) Exon 22 (G/C), Rs.362691, Polymorphism with Autism Spectrum Disorder among Iranian-Azeri Population

Authors

1 Assistant Professor of Cognitive Neuroscience. Department of Animal Biology Faculty of Natural Sciences. University of Tabriz, 29 Bahman Bolvard, Tabriz, Iran, IR.

2 Professor of Radiobiology, Department of Animal Biology Faculty of Natural Sciences. University of Tabriz, 29 Bahman Bolvard, Tabriz, Iran, IR.

3 Physiologist, Department of Animal Biology Faculty of Natural Sciences, University of Tabriz, 29 Bahman Bolvard, Tabriz, Iran, IR.

4 Child and Adolescent Psychiatrist MD, Research Centre of Psychiatry and Behavioral Science, Tabriz University of Medical Science, Tabriz, Iran, IR.

Abstract

Background
Autism spectrum disorder (ASD) is a intricate childhood neuropsychiatric disorder that is described by deficits in communication of verbal and non-verbal, reciprocal social interactions, stereotypic behaviors, interests, and activities. The studies of post-mortem neuro-anatomical anomalies have indicated that migration alterations could occur early during development (first trimester) in autistic brain. Since the Reelin gene, plays a crucial role in these migratory processes, it is subsequently considered as a potential candidate gene for autism.
Materials and Methods
In this case-control study, we recruited 74 patients with ASD and 88 healthy controls from Iranian-Azeri Population. Genomic DNA isolated from blood leukocytes of cases and control individuals by the proteinase K and using salt-out method. Single nucleotide polymorphisms (SNP) genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique.
Results
The allele and genotype frequencies did not show significant difference between autistic and control groups (P>0.05). No significant relationship was observed between the genders and genotypes in autism group (P>0.05).
Conclusion
The current study showed that the SNPs rs362691 could not be used as a useful molecular biomarker to predict genetic susceptibility for ASD among Iranian-Azeri patients.

Keywords