Assistant Professor of Cognitive Neuroscience. Department of Animal Biology Faculty of Natural Sciences. University of Tabriz, 29 Bahman Bolvard, Tabriz, Iran, IR.
Professor of Radiobiology, Department of Animal Biology Faculty of Natural Sciences. University of Tabriz, 29 Bahman Bolvard, Tabriz, Iran, IR.
Physiologist, Department of Animal Biology Faculty of Natural Sciences, University of Tabriz, 29 Bahman Bolvard, Tabriz, Iran, IR.
Child and Adolescent Psychiatrist MD, Research Centre of Psychiatry and Behavioral Science, Tabriz University of Medical Science, Tabriz, Iran, IR.
Autism spectrum disorder (ASD) is a intricate childhood neuropsychiatric disorder that is described by deficits in communication of verbal and non-verbal, reciprocal social interactions, stereotypic behaviors, interests, and activities. The studies of post-mortem neuro-anatomical anomalies have indicated that migration alterations could occur early during development (first trimester) in autistic brain. Since the Reelin gene, plays a crucial role in these migratory processes, it is subsequently considered as a potential candidate gene for autism.
Materials and Methods
In this case-control study, we recruited 74 patients with ASD and 88 healthy controls from Iranian-Azeri Population. Genomic DNA isolated from blood leukocytes of cases and control individuals by the proteinase K and using salt-out method. Single nucleotide polymorphisms (SNP) genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique.
The allele and genotype frequencies did not show significant difference between autistic and control groups (P>0.05). No significant relationship was observed between the genders and genotypes in autism group (P>0.05).
The current study showed that the SNPs rs362691 could not be used as a useful molecular biomarker to predict genetic susceptibility for ASD among Iranian-Azeri patients.