Cognitive Neuroscience, PhD. Department of Biology Faculty of Natural Sciences. University of Tabriz, 29 Bahman Bolvard, Tabriz, Iran, IR.
Physiology, MSc. Department of Biology Faculty of Natural Sciences. University of Tabriz, 29 Bahman Bolvard, Tabriz, Iran, IR.
Radiobiology, Prof. Department of Biology Faculty of Natural Sciences. University of Tabriz, 29 Bahman Bolvard, Tabriz, Iran.
Child and Adolescent Psychiatrist MD & Psychiatry assistant professor of Tabriz university of medical science, Tabriz, Iran.
Attention deficit hyperactivity disorder (ADHD), is a multifactorial disorder and converging evidence has implicated abnormalities of dopamine neurotransmission. The aim of this study was to examine the association of -141 polymorphisms in DRD2 gene with ADHA among Iranian-Azeri population.
Materials and Methods
A case–control association study included 153 patients with attention deficit hyper activity disorder (case group), and 133 healthy subjects (control group). Genomic DNA was extracted peripheral blood samples by salting-out method. Single nucleotide polymorphism (SNP) genotyping was performed by Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The data analysis was performed through Chi-square, with a significance level of 0.05.
Results: There was not significant difference in the allele and genotype frequencies between ADHD and -141C Ins/Del polymorphism in cases and controls (P>0.05). Ins/Ins homozygous dominants were more frequent in control group than the case group, but there was not significant difference observed (P>0.05). Del/Del homozygous dominants were not observed. No significant difference was detected in the allele and genotype frequencies between ADHD and -141 Insertion/Deletion polymorphism in cases and control groups (P>0.05).
Our results do not detected association between the -141C Ins/Del, rs1799732, polymorphism and ADHD disorder in population of Children in Iranian-Azeri.