Mobile phones and stethoscopes used in neonatology units could be colonized by potentiel bacteria pathogens. It can be a vector of severe nosocomial infections and multi-drug-resistant pathogens. The aim of this study is to evaluate the microbial contamination of mobile phones and stethoscopes, used by medical and paramedical staff. The study was conducted in neonatal intensive care unit (NICU) of Mohamed VI University Hospital, Marrakech (Morocco) in April 2016. The bacteriological study was made on 17 mobile phones and 13 stethoscopes. Samples were taken from all surfaces of mobile phones and stethoscopes, with a sterile swab. Bacterial contamination rate of all mobile phones and stethoscopes was 100%. The cultures of bacteria isolated were polymorphic. Among the bacteria isolated, six multi-resistant bacterial strains were isolated at the mobile phones (35%), corresponding to 4 Klebsiella pneumoniae and 2 strains of E. coli. A strain of Klebsiella pneumoniae multidrug-resistant (7.7%) was found on a stethoscope. This study shows that mobile phones and stethoscopes could be involoved in the transmission of severe nosocomial infections, with multidrug-resistance. As part of the prevention of such risks, we must educate the medical staff, users of mobile phones on the importance of hand washing and use of hydro-alcoholic solutions after each use of mobile phones and stethoscopes.